Sprint Interval Training (SIT) includes high intensity intermittent forms of cycling or running repeated sprints loads. Fatigue can be defined as impairment in muscle performance. Neuro-muscular and-or ionic perturbation during fatigue have investigated before. The aim of this study was to investigate the effects of SIT on sprint performance, ionic regulation and levels of creatine kinase (CK) and lactate dehydrogenase (LDH) during exhaustive exercise in young men. Fourteen male (22.3± 3.1 year) participated the study voluntarily. Before the training program, maximal 30 m sprint time and maximum voluntary contraction force (MVC) were measured. The subjects perform the exhaustion cycle test before and after the SIT. Earlobe capillary blood lactate concentration was measured just before and after the exhaustion test. The blood serum potassium (K+), inorganic phosphate (Pi), CK and LDH were measured just before and immediately after the exhaustion test from the antecubital vein. After the exhaustion test serum K+, Pi and lactate concentrations increased significantly (p<0.05). MVC, sprint performance, exhaustion time and exhaustion bicycle load and lactate concentration improved significantly after the SIT (p<0.05). It was concluded that SIT improved performance in very short training time. SIT improves against the exhaustion by endurance the fatigue; ion homeostasis and lactate transport capacity.