The mid gut bacterial flora contents of laboratory reared Anopheles pharoensis fed on either sugar or blood meal were quantitatively detected using facultative and/or aerobic bacterial cultures. Individual colonies (n = 11) were selected, subcultured and identified to the species level. Midgut bacterial counts of blood fed mosquitoes were higher than those of sugar fed ones. Bacterial identification revealed a complex flora with up to eight genera, in addition to other members not identified. Species most frequently isolated were Haemophillus parainfluenzae, Escherichia coli, Shigella sonnei, Serratia liquefaciens, Neisseria mucosa, Moraxella caviae, Staphylococcus aureus, Staphylococcus saprophyticus, Staphylococcus saccharolytics, Staphylococcus haemolyticus and Bacillus subtilis. GPT and GOT enzymes recorded high values during blood fed in symbiotic and aposymbiotic Anopheles than sugar fed ones. Also, protein, lipid and carbohydrate contents recorded high values in the same mosquitoes.

Keywords: Midgut, Bacteria, Anopheles, Acid phosphatase, Carbohydrates.