Petroleum waste is classified as hazardous and toxic waste. Types of petroleum hydrocarbon compounds are difficult degraded and toxic. One method which expected to degrade a complex material hydrocarbon to simplify substances is the phytoremediation technology. The performance of this technology can be improved by microbes isolates augmentation. The purpose of this study was to examine the potential of Rami (Boehmeria nivea L.) plant as a phytoremediator plant with isolates biofertilizer augmentation. The experiment used the randomized block design (RBD) with the factorial pattern was used in this. The first factor: biofertilizer isolates consisting of six genera: control treatment (without isolate biofertilizer; Acinetobacter sp. 2%; Pseudomonas sp. 2%; Penicillium sp. 2%, 2% mycorrhizal; and 2% isolate consortium. The second factor is the concentration of Azotobacter sp. which consists of three levels: Azotobacter sp. 0%, Azotobacter sp. 1% and Azotobacter sp. 2%. The results showed that the augmentation of the single isolate of biofertilizer in the phytoremediation process was not able to increase the rate of hydrocarbon degradation, and also the growth of hemp plants (Boehmeria nivea L.). But augmenting with consortium isolates tent to potentially increase the rate of degradation of petroleum hydrocarbons and plant height. AndAzotobacter sp. augmentation of 2% concentration increased the population of Phosphate solubilizing fungi (PSF) in the contaminated soil system.

Keywords: Augementation, Azotobacter sp., Boehmeria nivea L., Isolates, Petroleum, Phytoremediation,