Nowadays, every aspect of education, including the way we teach and learn, is being affected by technology, especially by the emergence of the Internet (Kung & Chuo, 2002). Since this technology tends to become an integral part of the modern world, it is used as a prevailing means for communication and education. In the meantime learners’ interest in ESP (English for Specific Purposes) has been reinforced by the popularity of the Internet. The Internet offers numerous authentic materials and ample opportunities to communicate in the foreign language. It fits into current theories of communicative language learning and learner autonomy (Davies, 2002). The present study aims to investigate the effect of teaching ESP materials through Web-based instruction and compare the result with that of the traditional text-based instruction. Meanwhile, the effect of selected ESP Web sites on ESP learning and factors contributing to learner autonomy are to be evaluated too. Hence, Data were collected form 82 students majoring in Nursing at Shahid Beheshti Medical Sciences University. A pre-test and a post-test on paramedical and Nursing-related texts were applied. The end results of the study revealed that the students had positive attitudes towards using the recommended Web sites in their ESP learning. Although there were some limitations such as lack of good access to the technology, the students found that learning ESP materials through Web sites was not only fun but also collaborative and student-centered.