The presence and concentration range of aflatoxin M1 (AFM1) were investigated by Enzyme Linked Immunosorbent Assay (ELISA) technique in 90 samples of raw ewe’s milk obtained from two dairies in Sanliurfa, Turkey. Analytical results showed that 88.9% of the samples were contaminated with AFM1. A total of 61.1% of the samples tested exceeded 50 ng/l set by the Turkish and European regulations for AFM1 in raw milk. AFM1 levels ranged from 17 to 232 ng/l and mean value was 89.89 ng/l. The incidence of AFM1 in milk from these dairies was 77.8 and 100% respectively. As a result, high incidence and levels of AFM1 in raw milk samples produced in Sanliurfa were determined.